Development of collagenous scaffolds for wound healing: characterization and in vivo analysis.

The development of wound dressings from biomaterials has been the subject of research due to their unique structural and functional characteristics. Proteins from animal origin, such as collagen and chitosan, act as promising materials for applications in injuries and chronic wounds, functioning as a repairing agent. This study aims to evaluate in vitro effects of scaffolds with different formulations containing bioactive compounds such as collagen, chitosan, N-acetylcysteine (NAC) and ε-poly-lysine (ε-PL). We manufactured a scaffold made of a collagen hydrogel bioconjugated with chitosan by crosslinking and addition of NAC and ε-PL. Cell viability was verified by resazurin and live/dead assays and the ultrastructure of biomaterials was evaluated by SEM. Antimicrobial sensitivity was assessed by antibiogram. The healing potential of the biomaterial was evaluated in vivo, in a model of healing of excisional wounds in mice. On the 7th day after the injury, the wounds and surrounding skin were processed for evaluation of biochemical and histological parameters associated with the inflammatory process. The results showed great cell viability and increase in porosity after crosslinking while antimicrobial action was observed in scaffolds containing NAC and ε-PL. Chitosan scaffolds bioconjugated with NAC/ε-PL showed improvement in tissue healing, with reduced lesion size and reduced inflammation. It is concluded that scaffolds crosslinked with chitosan-NAC-ε-PL have the desirable characteristics for tissue repair at low cost and could be considered promising biomaterials in the practice of regenerative medicine.

Revisiting the metallothionein genes polymorphisms and the risk of oral squamous cell carcinoma in a Brazilian population / Revisando los polimorfismos de los genes de metalotioneína y el riesgo de carcinoma oral de células escamosas en una población brasileña

Background: Metallothioneins (MTs) gene polymorphisms have been associated with the ability of free radicalscavenging and detoxification of heavy metals leading to cancer development. Our aim was to revisit, in a Brazil-ian population, single-nucleotide polymorphisms (SNPs) of the MT gene family previously associated with oralsquamous cell carcinoma (OSCC).Material and Methods: A case-control investigation with 28 OSCC patients and 45 controls was conducted, usingconventional risk factors (tobacco use and alcohol consumption) as covariates. SNPs genotyping for rs8052334(MT1B), rs964372 (MT1B), and rs1610216 (MT2A) was performed by PCR-RFLP, and SNPs for rs11076161(MT1A) were analyzed by TaqMan assay.Results: The only SNP associated with increased risk for OSCC was the MT-1A AA genotype (OR = 4.7; p = 0.01).We have also evidenced for the first time a significant linkage disequilibrium between the SNPs of MT-2A andMT-1A in this population with the highest frequency (30%) of the unfavorable haplotype G/A/C/T (rs1610216 /rs11076161 / rs964372 / rs8052334) of MT gene polymorphisms (OR = 6.2; p = 0.04). Interestingly, after removingthe effects of conventional risk factors, we have uncovered the significance of the AA genotype of the rs11076161with increased odds of 19-fold higher towards OSCC development.Conclusions: This is the first demonstration that a significant linkage disequilibrium among gene polymor-phisms of the MT family may affect susceptibility to oral cancer, which is conditioned by the G/A/C/T haplotype(rs1610216/rs11076161/rs964372/ rs8052334) and the MT-1A gene polymorphism has a potential clinical utility forthe OSCC risk assessment.(AU)

Simple bone cyst: description of 60 cases seen at a Brazilian School of Dentistry and review of international literature

BACKGROUND: The aim of this study was to describe the relative frequency and the main demographic and clinic-radiographic features related to patients diagnosed with Simple bone cyst (SBC) in an Oral Diagnosis Service in Southeast Brazil and present a review and discussion of international literature on this topic. MATERIAL AND METHODS: SBC cases from our service encompassing the period between 1978 and 2017 were selected. In addition, a literature search was performed in the Pubmed/MEDLINE online electronic database published between 1951 and 2019. RESULTS: A total of 2,459 cystic lesions were documented in our service, thus 60 patients were diagnosed with the SBC representing 2.4% of all jaw cystic. Most of cases were asymptomatic. Multiple SBC lesions were seen in two patients (3.4%) and association with cemento-osseous dysplasia was seen in one female patient (1.7%). A total of 793 cases were enrolled in this literature review. CONCLUSIONS: The SBC is an asymptomatic lesion often discovered in routine image exams in young patients. The unilocular, well defined margin with scalloped appearance is characteristic and helps the definition of diagnosis. This review suggests a different epidemiologic trend concerning to the sex and it confirms the posterior region of mandible as the more frequent location. The conservative treatment with limited exploration and curettage remains as the gold-standard treatment

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Differential metallothionein expression in oral lichen planus and amalgam-associated oral lichenoid lesions

BACKGROUND: Oral lichen planus (OLP) is a chronic inflammatory disease mediated by T cells, which manifests as reticular (white) or erosive (red) lesions, that are eventually painful. Oral lichenoid lesion (OLL) are distinguished from OLP by the presence of precipitating factors. The aim of this study was to evaluate whether the presence of metallothionein, which is involved in anti-apoptotic pathways and the anti-oxidative response, could serve as a differential diagnostic for OLP and OLL. MATERIAL AND METHODS: We evaluated the expression of metallothionein in 40 cases of OLP and 20 cases of OLL using immunohistochemistry. Results and CONCLUSIONS: White OLP has higher concentrations of metallothionein than red OLP in basal and parabasal layers. Moreover, metallothionein was more frequently observed in the cytoplasm and nuclei of basal cells in OLP patients compared to the same regions of OLL cases. Metallothionein levels are related to OLP severity and may contribute to a differential diagnosis between OLP and OLL

Primary amelanotic mucosal melanoma of the oronasal region: report of two new cases and literature review.

Primary amelanotic mucosal melanoma is a rare entity with challenging histopathological features. Because these tumors are thought to be biologically more aggressive, they have a poorer prognosis than that of pigmented melanomas. In this work, we present a literature review about the clinical, histopathological, and immunohistochemical features of primary amelanotic mucosal melanoma of the oronasal region and report two new cases. Amelanotic mucosal melanoma commonly affects men in the seventh decade of life and tend to have a poor prognosis, as seen by the high incidence of metastasis, recurrences, and, ultimately, death. There is a similar pattern in the clinic-pathological predilections (such as age, gender, primary site, and metastatic potential) of amelanotic mucosal melanoma when comparing with data reported for pigmented lesions. This work reinforces knowledge about amelanotic mucosal melanomas and epidemiologic predilections. The optimal management of this lesion remains controversial.

Stevens-Johnson syndrome: an oral viewpoint.

The Stevens-Johnson syndrome (SJS) is a severe variant of erythema multiforme (EM) that occasionally can rise to systemic upset and possibly compromise life. In this report, we described important oral and dermatologic aspects of a case of SJS triggered by Mycoplasma pneumoniae infection.

Perfil estomatológico de pacientes psoriátricos: análise de 50 casos / Stomatologic feactures in psoriatric patients: analysis of 50 cases

Introdução: a psoríase é uma doença dermatológica crônica que acomete cerca de 3% da população mundial e pode representar um fator importante de exclusão social. A manifestação bucal ainda é um assunto controverso na literatura sendo descrita como lesões brancas, eritematosas, úlceras e papuloescamosa. Objetivos - Avaliar a presença de manifestações bucais da psoríase, considerando-se a localização e as características clínicas das lesões encontradas, o sexo, a idade e a raça dos acometidos. Métodos - Estudo observacional, do tipo transversal, com pacientes que procuraram atendimento no ambulatório de dermatologiado Hospital de Clínicas da Universidade Federal de Uberlândia (HCUFU) entre dezembro de 2006 a novembro de 2007 (n=50). Resultados - A idade variou entre 7 e 78 anos com media de idade de 43,58 anos e mediana de 45 anos. Não houve predileção por sexo, sendo que 26 pacientes eram do sexo masculino e 24 feminino. No exame bucal foram encontradas lesões em 44% dos pacientes, sendo 16 lesões em língua (53,3%) na forma de fissuras ou áreas despapiladas, 2 em mucosa jugal (6,7%) na forma de placas brancas, 5 em lábio (16,7%) na forma de queilites e ressecamento e 7 lesões eritematosa sem palato (23,3%). Conclusões - Não foi possível identificar alteração que pudesse estar associada à psoríase, visto que nenhuma lesão acompanhou o quadro da doença cutâneaou teve diagnóstico histológico confirmado. Com relação às alterações em língua, tais como língua fissurada e língua geográfica, a incidência encontrada foi muito próxima a outros estudos em pacientes sem uma doença de base importante.

Introduction: psoriasis is a chronic dermatologic disease that affects at least 3% of the world population. Oral manifestation is still a controversy in the literature. It has been described by some authors in clinical case reports as white or erythematous lesions, ulcers and descamative papules. Objectives - To evaluate the presence of oral manifestations associated with psoriasis, considering location and clinical characteristics of the lesions and also gender, age and race of patients. Method - It was an observational,cross-sectional study of patients who sought for treatment at the Dermatology Service ofthe University Hospital (Uberlândia Federal University); between December 2006 and November 2007 (n=50) . Results - Age varied from 7 to 78 years, mean age of 43,58years and median of 45 years. There was no predominance of male (n=26) or female(n=24) patients. Lesions were found in 44% of patients, 16 (53,3%) of these lesions werein tongue, 2 (6,7%) white plaques in the cheeks, 5 (16,7%) lesion in lips as cheilitis anddryness and 7 (23,3%) erythematous areas in the hard palate. Conclusions - After all,it was not possible to make any specific association between oral lesions and psoriasis because the lesions did not follow the cutaneous disease stage nor was histologically confirmed. Also the tongue alterations incidence identified in this study was similar tothat found in population without other diseases.